MAD2L1 anticorps

N° du produit ABIN129510

N° du produit (Fournisseur): 200-301-461

Cet anticorps anti-MAD2L1 est un anticorps Souris Monoclonal détectant MAD2L1 dans WB, ELISA et IP. Adapté pour Humain.

Aperçu rapide pour MAD2L1 anticorps (ABIN129510)

Antigène: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAD2L1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 17D10

Détail du produit anti-MAD2L1 anticorps

N° du produit (Fournisseur): 200-301-461

Fournisseur: Rockland

Fonction: MAD2L1 Antibody

Réactivité croisée (Details): This Protein A purified antibody is directed against human MAD2L1 protein.

Attributs du produit: Synonyms: mouse anti-MAD2L1 antibody, Mitotic arrest deficient yeast homolog like 1 antibody, Mitotic spindle assembly checkpoint protein antibody

Purification: The product was purified from tissue culture supernatant by chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with full-length recombinant human MAD2L1 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG1

Information d'application

Indications d'application:

Application Note: This protein A purified antibody has been tested for use in immunoprecipitation and by western blot.  This antibody is not applicable for immunofluorescence microscopy.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur MAD2L1

Antigène: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Autre désignation: MAD2L1

Sujet: Background: MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. This protein has a nuclear localization.  A MAD2 pseudogene has been mapped to chromosome 14.

ID gène: 4085, 4505067

UniProt: Q13257